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XE242 Mouse CK2alpha CS2p+
(Plasmid #16730)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16730 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2P+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse Casein Kinase II alpha-pCS2P+
  • Alt name
    casein Kinase
  • Alt name
    ck2
  • Alt name
    ck2alpha
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Entrez Gene
    Csnk2a1 (a.k.a. RP23-452D3.3, Csnk2a1-rs4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Stu1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Subcloned from XE 163 into Stu/Xba sites of CS2P+
Linearize with ASP718 and transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE242 Mouse CK2alpha CS2p+ was a gift from Randall Moon (Addgene plasmid # 16730 ; http://n2t.net/addgene:16730 ; RRID:Addgene_16730)

  • For your References section:

    Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Waxman JS, Hocking AM, Stoick CL, Moon RT. Development. 2004 Dec. 131(23):5909-21. 10.1242/dev.01520 PubMed 15539487
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