pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2
(Plasmid #167295)

• Purpose: Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry
• Depositing Lab: Agnel Sfeir
• Publication: Tigano et al Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w. Epub 2021 Feb 24.

Backbone

• Vector backbone: PX458
• Total vector size (bp): 9720
• Modifications to backbone: Inserted a second U6 gRNA expression cassette
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BAK
• gRNA/shRNA sequence: GCATGAAGTCGACCACGAAG, GGCCATGCTGGTAGACGTGT
• Species: H. sapiens (human)
• Entrez Gene: BAK1 (a.k.a. BAK, BAK-LIKE, BCL2L7, CDN1)

Resource Information

• Supplemental Documents:
  • pSpCas9nucl-BAK_g1-BAK_g2-T2A_mCherry
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.01.31.929075v1 for bioRxiv preprint.