pLenti-RIGI-Apex- T2A-EGFP
(Plasmid #167288)

• Purpose: Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.
• Depositing Lab: Agnel Sfeir
• Publication: Tigano et al Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w. Epub 2021 Feb 24.

Backbone

• Vector backbone: pLENTI
• Total vector size (bp): 7358
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: eGFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DDX58
• Alt name: RIG-I
• Species: H. sapiens (human)
• Insert Size (bp): 3528
• Entrez Gene: RIGI (a.k.a. DDX58, RIG-I, RIG1, RLR-1, SGMRT2)
• Tags / Fusion Proteins:
  • APEX2 (N terminal on insert)
  • EGFP (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • pLenti_RIGI-Apex_T2A-EGFP.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.01.31.929075v1 for bioRxiv preprint.