XE244 Mouse Naked-1 CS2p+
(Plasmid #16728)

• Depositing Lab: Randall Moon
• Publication: Waxman et al Development. 2004 Dec. 131(23):5909-21.

Backbone

• Vector backbone: pCS2P+
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mouse Naked-1
• Alt name: nkd1
• Species: M. musculus (mouse)
• Entrez Gene: Nkd1 (a.k.a. 2810434J10Rik, 9030215G15Rik, Nkd)
• Tags / Fusion Proteins:
  • myc (C terminal on insert)
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba1 (unknown if destroyed)
• 3′ cloning site: Xho1 (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Description: Subcloned Keith Wharton pCDNA3 insert into Xba (blunted) and Xho sites of CS2P+
Linearize with Sac2 and transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.

Addgene QC found an E431K mutation that is not known to affect function.