pET51b-Strep-hAGT-His
(Plasmid
#167276)
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PurposeOverexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167276 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET51b(+)
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehAGT
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SpeciesH. sapiens (human)
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Insert Size (bp)744
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Entrez GeneAGT (a.k.a. ANHU, SERPINA8, hFLT1)
- Promoter T7
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Tags
/ Fusion Proteins
- Strep-tag (N terminal on insert)
- His x10 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439540v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET51b-Strep-hAGT-His was a gift from Kai Johnsson (Addgene plasmid # 167276 ; http://n2t.net/addgene:167276 ; RRID:Addgene_167276) -
For your References section:
Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Wilhelm J, Kuhn S, Tarnawski M, Gotthard G, Tunnermann J, Tanzer T, Karpenko J, Mertes N, Xue L, Uhrig U, Reinstein J, Hiblot J, Johnsson K. Biochemistry. 2021 Aug 24;60(33):2560-2575. doi: 10.1021/acs.biochem.1c00258. Epub 2021 Aug 2. 10.1021/acs.biochem.1c00258 PubMed 34339177