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Addgene

XE245 Mouse Naked-2 CS2p+
(Plasmid #16727)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16727 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCS2P+P
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mouse Naked-2 pCS2P+F
  • Alt name
    nkd2
  • Species
    M. musculus (mouse)
  • Mutation
    *
  • Entrez Gene
    Nkd2 (a.k.a. 2210403L10Rik, AW212591)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (destroyed during cloning)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Subcloned Keith Wharton pCDNA3 insert into Xba (blunted) and Xho sites of CS2P+

Linearize with Sac2 and transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.

* Addgene's QC finds a 163bp deletion at the 3'end when compared to the NCBI reference sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE245 Mouse Naked-2 CS2p+ was a gift from Randall Moon (Addgene plasmid # 16727 ; http://n2t.net/addgene:16727 ; RRID:Addgene_16727)
  • For your References section:

    Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes. Waxman JS, Hocking AM, Stoick CL, Moon RT. Development. 2004 Dec. 131(23):5909-21. 10.1242/dev.01520 PubMed 15539487