XE245 Mouse Naked-2 CS2p+
(Plasmid #16727)

• Depositing Lab: Randall Moon
• Publication: Waxman et al Development. 2004 Dec. 131(23):5909-21.

Backbone

• Vector backbone: pCS2P+P
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mouse Naked-2 pCS2P+F
• Alt name: nkd2
• Species: M. musculus (mouse)
• Mutation: *
• Entrez Gene: Nkd2 (a.k.a. 2210403L10Rik, AW212591)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xba1 (destroyed during cloning)
• 3′ cloning site: Xho1 (unknown if destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Description: Subcloned Keith Wharton pCDNA3 insert into Xba (blunted) and Xho sites of CS2P+

Linearize with Sac2 and transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.

* Addgene's QC finds a 163bp deletion at the 3'end when compared to the NCBI reference sequence