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Addgene

pMCSG53_Nsp3_Y_2
(Plasmid #167262)

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Item Catalog # Description Quantity Price (USD)
Plasmid 167262 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pMCSG53
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nsp3 (residues 1619-1945)
  • Alt name
    Y1 or CoV-Y domain
  • Species
    SARS-CoV-2
  • Mutation
    none
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • His6-TEV site (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCSG53_Nsp3_Y_2 was a gift from Alexei Savchenko (Addgene plasmid # 167262 ; http://n2t.net/addgene:167262 ; RRID:Addgene_167262)
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