XE246 Xenopus CK1 epsilon D to N CS2+
(Plasmid #16726)

• Depositing Lab: Randall Moon
• Publication: Waxman et al Development. 2004 Dec. 131(23):5909-21.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: casein kinase 1, epsilon
• Alt name: ck1
• Alt name: xck1
• Species: X. laevis (frog)
• Mutation: D128N
• Entrez Gene: csnk1a1 (a.k.a. ck1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: From John Graff, in Peters et al 1999
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Description: From John Graff, in Peters et al 1999.

Notes for Use: Select clones with amp.
Reference: used in Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.