Skip to main content
Addgene

pMCSG53_Nsp1
(Plasmid #167256)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 167256 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pMCSG53
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nsp1 (residues 13-127)
  • Species
    SARS-CoV-2
  • Mutation
    none
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • His6-TEV site (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCSG53_Nsp1 was a gift from Alexei Savchenko (Addgene plasmid # 167256 ; http://n2t.net/addgene:167256 ; RRID:Addgene_167256)

  • For your References section:

    Structural characterization of nonstructural protein 1 from SARS-CoV-2. Semper C, Watanabe N, Savchenko A. iScience. 2021 Jan 22;24(1):101903. doi: 10.1016/j.isci.2020.101903. Epub 2020 Dec 7. 10.1016/j.isci.2020.101903 PubMed 33319167
Related items: