pJBL7061
(Plasmid
#167230)
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Purposecell-free cascaded lead sensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 167230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepPbr-AKSIRV
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SpeciesOther
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Tag
/ Fusion Protein
- 6X His (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJBL7061 was a gift from Julius Lucks (Addgene plasmid # 167230 ; http://n2t.net/addgene:167230 ; RRID:Addgene_167230) -
For your References section:
At-Home, Cell-Free Synthetic Biology Education Modules for Transcriptional Regulation and Environmental Water Quality Monitoring. Jung JK, Rasor BJ, Rybnicky GA, Silverman AD, Standeven J, Kuhn R, Granito T, Ekas HM, Wang BM, Karim AS, Lucks JB, Jewett MC. ACS Synth Biol. 2023 Oct 20;12(10):2909-2921. doi: 10.1021/acssynbio.3c00223. Epub 2023 Sep 12. 10.1021/acssynbio.3c00223 PubMed 37699423