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Addgene

lentiCRISPR-v2-Blast-Puro
(Plasmid #167186)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167186 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCRISPR v2 (Addgene #52961)
  • Backbone size w/o insert (bp) 14873
  • Modifications to backbone
    Replaced the Cas9 sequence in lentiCRISPR v2 plasmid (Addgene, 52961) with blasticidin resistance gene
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Blasticidin S deaminase
  • Insert Size (bp)
    396

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lentiCRISPR-v2-Blast-Puro was a gift from Yin Shen (Addgene plasmid # 167186 ; http://n2t.net/addgene:167186 ; RRID:Addgene_167186)

  • For your References section:

    Parallel characterization of cis-regulatory elements for multiple genes using CRISPRpath. Ren X, Wang M, Li B, Jamieson K, Zheng L, Jones IR, Li B, Takagi MA, Lee J, Maliskova L, Tam TW, Yu M, Hu R, Lee L, Abnousi A, Li G, Li Y, Hu M, Ren B, Wang W, Shen Y. Sci Adv. 2021 Sep 17;7(38):eabi4360. doi: 10.1126/sciadv.abi4360. Epub 2021 Sep 15. 10.1126/sciadv.abi4360 PubMed 34524848
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