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Addgene

S+12subs
(Plasmid #167177)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167177 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac Dual
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 5193
  • Total vector size (bp) 9177
  • Vector type
    Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Please note XL10-Gold used in this study
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ATP1A1-S+12R
  • Alt name
    ATP1A1
  • Alt name
    Na+K+ATPase alpha 1
  • Species
    Leptodactylus latrans
  • Insert Size (bp)
    3069
  • Mutation
    Changed Q at 111 to R; A at 112 to T; E at 116to D; N at 122 to D; I at 135 to V; L at 180 to Q; I at 199 to L; I at 279 to V; S at 403 to C; L at 536 to M; Q at 701 to L; I at 788 to M
  • Promoter PH

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer ATTCCGGATTATTCATACCGTCCCACCATCG
  • 3′ sequencing primer GTGGTATGGCTGATTATGATCCTCTAGTACTTCTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ATP1B1
  • Alt name
    Na+K+ATPase beta 1
  • Species
    Leptodactylus latrans
  • Insert Size (bp)
    917
  • Promoter P10

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer CGGGTTCCTTCCGGTATTGTCTCCTTC
  • 3′ sequencing primer ACGGACCTTTAATTCAACCCAACACAATATA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    S+12subs was a gift from Susanne Dobler (Addgene plasmid # 167177 ; http://n2t.net/addgene:167177 ; RRID:Addgene_167177)

  • For your References section:

    Concerted evolution reveals co-adapted amino acid substitutions in Na(+)K(+)-ATPase of frogs that prey on toxic toads. Mohammadi S, Yang L, Harpak A, Herrera-Alvarez S, Del Pilar Rodriguez-Ordonez M, Peng J, Zhang K, Storz JF, Dobler S, Crawford AJ, Andolfatto P. Curr Biol. 2021 Jun 21;31(12):2530-2538.e10. doi: 10.1016/j.cub.2021.03.089. Epub 2021 Apr 21. 10.1016/j.cub.2021.03.089 PubMed 33887183
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