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pEGFP ratAPOBEC1 L173A/G227A
(Plasmid #167169)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167169 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3943
  • Total vector size (bp) 4627
  • Modifications to backbone
    the EGFP cds has been removed
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    APOBEC1
  • Alt name
    apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    603
  • Mutation
    changed Leucine 173 to Alanine; changed Glycine 227 to Alanine
  • GenBank ID
    NM_012907
  • Entrez Gene
    Apobec1 (a.k.a. REPR, apobec-1)
  • Promoter CMV promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CGTGTACGGTGGGAGGTCTA
  • 3′ sequencing primer TTCAGGTTCAGGGGGAGGTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP ratAPOBEC1 L173A/G227A was a gift from Silvestro Conticello (Addgene plasmid # 167169 ; http://n2t.net/addgene:167169 ; RRID:Addgene_167169)

  • For your References section:

    Dimerisation of APOBEC1 is dispensable for its RNA editing activity. Chieca M, Montini M, Severi F, Pecori R, Conticello S. bioRxiv 2021 10.1101/410803
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