pDONR221_SMAD2_HAe2_Bsd
(Plasmid #167166)

• Purpose: Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.
• Depositing Lab: Alexander Loewer
• Publication: pDONR221_SMAD2_e2_Bsd (unpublished)

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2471
• Total vector size (bp): 4762
• Vector type: Repair template for gene editing in human cells
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette
• Species: H. sapiens (human)
• Insert Size (bp): 2325
• GenBank ID: NG_029946.1
• Entrez Gene: SMAD2 (a.k.a. CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2)
• Tag / Fusion Protein:
  • blasticidin

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• Supplemental Documents:
  • pDONR221_SMAD2_HAe2_Bsd.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry