Skip to main content
Addgene

pgRNA_CV2
(Plasmid #167165)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167165 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    gRNA_Cloning Vector (Plasmid #41824)
  • Backbone manufacturer
    Depositing Lab: George Church
  • Backbone size (bp) 3914
  • Modifications to backbone
    AgeI site was added to simplify cloning by Gibson assembly
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter U6 promoter

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pgRNA_CV2 was a gift from Alexander Loewer (Addgene plasmid # 167165 ; http://n2t.net/addgene:167165 ; RRID:Addgene_167165)

  • For your References section:

    PCNA-Mediated Degradation of p21 Coordinates the DNA Damage Response and Cell Cycle Regulation in Individual Cells. Sheng C, Mendler IH, Rieke S, Snyder P, Jentsch M, Friedrich D, Drossel B, Loewer A. Cell Rep. 2019 Apr 2;27(1):48-58.e7. doi: 10.1016/j.celrep.2019.03.031. 10.1016/j.celrep.2019.03.031 PubMed 30943414
Related items: