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pPPAT-mCherry
(Plasmid #167162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Total vector size (bp) 6250
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPAT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1551
  • Entrez Gene
    PPAT (a.k.a. ATASE, GPAT, PRAT)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPPAT-mCherry was a gift from Stephen Benkovic (Addgene plasmid # 167162 ; http://n2t.net/addgene:167162 ; RRID:Addgene_167162)

  • For your References section:

    Detecting Purinosome Metabolon Formation with Fluorescence Microscopy. Pedley AM, Benkovic SJ. Methods Mol Biol. 2018;1764:279-289. doi: 10.1007/978-1-4939-7759-8_17. 10.1007/978-1-4939-7759-8_17 PubMed 29605921
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