pPPAT-mCherry
(Plasmid
#167162)
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PurposeExpresses human PPAT-mCherry fusion in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4722
- Total vector size (bp) 6250
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPAT
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SpeciesH. sapiens (human)
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Insert Size (bp)1551
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPPAT-mCherry was a gift from Stephen Benkovic (Addgene plasmid # 167162 ; http://n2t.net/addgene:167162 ; RRID:Addgene_167162) -
For your References section:
Detecting Purinosome Metabolon Formation with Fluorescence Microscopy. Pedley AM, Benkovic SJ. Methods Mol Biol. 2018;1764:279-289. doi: 10.1007/978-1-4939-7759-8_17. 10.1007/978-1-4939-7759-8_17 PubMed 29605921