M7 pdnPKA-GFP
(Plasmid #16716)

• Depositing Lab: Randall Moon
• Publication: Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi: 10.1006/dbio.1996.0209.

Backbone

• Vector backbone: CS2+
• Backbone size w/o insert (bp): 4100
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dnPKA
• Alt name: Protein Kinase A
• Alt name: PKa
• Species: M. musculus (mouse)
• Mutation: Dominant negative. Mutations at cAMP binding sites.
• Entrez Gene: Prkar1a (a.k.a. 1300018C22Rik, RIalpha, Tse-1, Tse1)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: dominant negative Protein Kinase A-bright green flourescent protein fusion protein.
To make sense RNA, for expression, cut with Not1 and transcribe with SP6.

Plasmid History:
Constructed by Anne Ungar in 1996.

Citation: Ungar and Moon (1996)
Developmental Biology 178:186-191.