pX037
(Plasmid #167156)

• Purpose: MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.
• Depositing Lab: Karen Sarkisyan
• Publication: Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 10.1038/s41587-020-0500-9.

Backbone

• Vector backbone: Level2-like
• Backbone size w/o insert (bp): 4839
• Vector type: Plant Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: caffeic acid cycle genes
• Species: Escherichia coli, Neonothopanus nambi
• Insert Size (bp): 15128
• Promoter: pNOS, p35s

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Synthetic
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Encodes 5 transcription units for expression in plants: Kanamycin resistance cassette, Neonothopanus nambi hispidin synthase, Neonothopanus nambi hispidin-3-hydroxylase, Neonothopanus nambi luciferase, Neonothopanus nambi caffeoylpiruvate hydrolase

The depositing lab does not recommend this plasmid for most applications, and recommend using pNK3071 instead (https://www.addgene.org/219755/)