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Addgene

pBJ2
(Plasmid #167131)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167131 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBRMCS-5
  • Backbone manufacturer
    Kenneth M. Peterson
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    luxCDABE
  • Species
    Photorhabdus luminescens
  • Insert Size (bp)
    7600
  • Promoter Kanamycin promoter

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    tnsABCD and luxCDABE genes were amplified from pBEN276 (Addgene plasmid #69150).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBJ2 was a gift from Akira Mine (Addgene plasmid # 167131 ; http://n2t.net/addgene:167131 ; RRID:Addgene_167131)

  • For your References section:

    A versatile Tn7 transposon-based bioluminescence tagging tool for quantitative and spatial detection of bacteria in plants. Matsumoto A, Schluter T, Melkonian K, Takeda A, Nakagami H, Mine A. Plant Commun. 2021 Jul 20;3(1):100227. doi: 10.1016/j.xplc.2021.100227. eCollection 2022 Jan 10. 10.1016/j.xplc.2021.100227 PubMed 35059625
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