pET21b-RBD-JA1
(Plasmid #167126)

• Purpose: Expresses SARS-CoV-2 RBD in bacterial system
• Depositing Lab: David Margulies
• Publication: Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.01.27.428466.

Backbone

• Vector backbone: pET21b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5442
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 Receptor Binding domain
• Species: SARS-CoV-2
• Insert Size (bp): 596
• Mutation: residues (333 to 529 aa)
• GenBank ID: YP_009724390.1
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 Fwd
• 3′ sequencing primer: T7 Term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cloned from Addgene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry