p35S_GFP-GUS
(Plasmid
#167122)
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PurposePlant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepB7m34GW
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 8000
- Total vector size (bp) 11934
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Vector typePlant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP-GUS
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Alt namemGFP5
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Alt namebeta-glucuronidase
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Insert Size (bp)2562
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GenBank ID
- Promoter CaMV 35S
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Tag
/ Fusion Protein
- mGFP5, GUS
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TTCGACGGAGAAGGTGACGATAC
- 3′ sequencing primer TTGGTGAAGATATCCGCCAACTG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p35S_GFP-GUS was a gift from Arturo Marí-Ordóñez & Olivier Voinnet (Addgene plasmid # 167122 ; http://n2t.net/addgene:167122 ; RRID:Addgene_167122) -
For your References section:
A genome-wide transcriptome and translatome analysis of Arabidopsis transposons identifies a unique and conserved genome expression strategy for Ty1/Copia retroelements. Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A. Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. Epub 2017 Aug 7. 10.1101/gr.220723.117 PubMed 28784835