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Addgene

p35S_EVDm1
(Plasmid #167121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pB7m34GW
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 13470
  • Vector type
    Plant Expression
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EVADE (EVD)
  • Alt name
    Copia93
  • Alt name
    AT5TE20395
  • Alt name
    AT5G17125
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    4215
  • Mutation
    mutated four nucleotides at the snoRNA U1 binding site from the 5'splice donor site to prevent splicing
  • GenBank ID
    NC_003076.8
  • Entrez Gene
    AT5G17125 (a.k.a. AT5G17125)
  • Promoter CaMV 35S

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TTCGACGGAGAAGGTGACGATAC
  • 3′ sequencing primer TTGGTGAAGATATCCGCCAACTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p35S_EVDm1 was a gift from Arturo Marí-Ordóñez & Olivier Voinnet (Addgene plasmid # 167121 ; http://n2t.net/addgene:167121 ; RRID:Addgene_167121)

  • For your References section:

    A genome-wide transcriptome and translatome analysis of Arabidopsis transposons identifies a unique and conserved genome expression strategy for Ty1/Copia retroelements. Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A. Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. Epub 2017 Aug 7. 10.1101/gr.220723.117 PubMed 28784835
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