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XE237 Human LEF-1 pCS2+
(Plasmid #16709)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16709 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human LEF-1
  • Alt name
    hlef1
  • Alt name
    lef1
  • Species
    H. sapiens (human)
  • Entrez Gene
    LEF1 (a.k.a. LEF-1, TCF10, TCF1ALPHA, TCF7L3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Stu1 (destroyed during cloning)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: This is a wild type LEF-1, cloned into pCS2+.

Reference: Made by Ken-Ichi Takemaru. It is an Eco-R1-blunted HindIII fragment cloned into EcoR1/Stu1 sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE237 Human LEF-1 pCS2+ was a gift from Randall Moon (Addgene plasmid # 16709 ; http://n2t.net/addgene:16709 ; RRID:Addgene_16709)

  • For your References section:

    Lymphoid enhancer factor-1 links two hereditary leukemia syndromes through core-binding factor alpha regulation of ELA2. Li FQ, Person RE, Takemaru K, Williams K, Meade-White K, Ozsahin AH, Gungor T, Moon RT, Horwitz M. J Biol Chem. 2004 Jan 23;279(4):2873-84. doi: 10.1074/jbc.M310759200. Epub 2003 Oct 31. 10.1074/jbc.M310759200 PubMed 14594802
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