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Addgene

XE90 pCS2P+ XCtBP
(Plasmid #16704)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2P+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XCtBP
  • Alt name
    ctbp
  • Alt name
    tcf
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2000
  • Entrez Gene
    ctbp2 (a.k.a. ctbp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid History:
Constructed by Jeff Brown.

Notes for Use:
The ~2 kb dropout of the Sal I digest of XTcf-3 interacting clone 2-1 (in pGBT9) was ligated into the XhoI site of pCS2P+. For CS2+MT XCtBP, the Sal I dropout was blunted with Klenow and cloned into the StuI site of pCS2+MT.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE90 pCS2P+ XCtBP was a gift from Randall Moon (Addgene plasmid # 16704 ; http://n2t.net/addgene:16704 ; RRID:Addgene_16704)
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