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pInducer21-3xFlag-S208C-MEFV
(Plasmid #167019)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pInducer21
  • Backbone size w/o insert (bp) 10550
  • Total vector size (bp) 12974
  • Vector type
    Lentiviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MEFV
  • Alt name
    pyrin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2424
  • Mutation
    Serine 208 changed to Cysteine
  • Entrez Gene
    MEFV (a.k.a. FMF, MEF, PAAND, TRIM20)
  • Promoter CMV Dox inducible
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pInducer21-3xFlag-S208C-MEFV was a gift from Thomas Henry (Addgene plasmid # 167019 ; http://n2t.net/addgene:167019 ; RRID:Addgene_167019)

  • For your References section:

    Functional Assessment of Disease-Associated Pyrin Variants. Chirita D, Jamilloux Y, Henry T, Magnotti F. Methods Mol Biol. 2022;2523:179-195. doi: 10.1007/978-1-0716-2449-4_12. 10.1007/978-1-0716-2449-4_12 PubMed 35759198
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