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pENTR1A-3XFlag-WT-MEFV
(Plasmid #167018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR1A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3200
  • Total vector size (bp) 4710
  • Modifications to backbone
    Deletion of the CcdB-CmR cassette Addition of : Kpn1-3xFlag-Not1-MEFV-Xho1
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MEFV
  • Alt name
    Pyrin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2424
  • Entrez Gene
    MEFV (a.k.a. FMF, MEF, PAAND, TRIM20)
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

the pENTR1A backbone might impose some restriction

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR1A-3XFlag-WT-MEFV was a gift from Thomas Henry (Addgene plasmid # 167018 ; http://n2t.net/addgene:167018 ; RRID:Addgene_167018)

  • For your References section:

    Functional Assessment of Disease-Associated Pyrin Variants. Chirita D, Jamilloux Y, Henry T, Magnotti F. Methods Mol Biol. 2022;2523:179-195. doi: 10.1007/978-1-0716-2449-4_12. 10.1007/978-1-0716-2449-4_12 PubMed 35759198
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