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Addgene

pDONR221-SARS-CoV-2 S1 RBD
(Plasmid #167013)

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Item Catalog # Description Quantity Price (USD)
Plasmid 167013 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDONR221
  • Backbone size w/o insert (bp) 4762
  • Total vector size (bp) 5434
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    S
  • Species
    SARS-CoV-2 isolate Wuhan-Hu-1
  • Insert Size (bp)
    672
  • Mutation
    No stop codon
  • GenBank ID
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cloned from pDONR223 SARS-CoV-2 S (Addgene #149329) received from Addgene

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-SARS-CoV-2 S1 RBD was a gift from Spyros Petrakis (Addgene plasmid # 167013 ; http://n2t.net/addgene:167013 ; RRID:Addgene_167013)

  • For your References section:

    AI Promoted Virtual Screening, Structure-Based Hit Optimization, and Synthesis of Novel COVID-19 S-RBD Domain Inhibitors. Gkekas I, Katsamakas S, Mylonas S, Fotopoulou T, Magoulas GEpsilon, Tenchiu AC, Dimitriou M, Axenopoulos A, Rossopoulou N, Kostova S, Wanker EE, Katsila T, Papahatjis D, Gorgoulis VG, Koufaki M, Karakasiliotis I, Calogeropoulou T, Daras P, Petrakis S. J Chem Inf Model. 2024 Nov 13. doi: 10.1021/acs.jcim.4c01110. 10.1021/acs.jcim.4c01110 PubMed 39535926
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