pDONR221-ACE2 receptor 19-615
(Plasmid #167012)

• Purpose: Gateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2
• Depositing Lab: Spyros Petrakis
• Publication: Gateway entry clones of SARS-CoV-2 Spike S1 RBD domain and interacting region of ACE2 receptor (unpublished)

Backbone

• Vector backbone: pDONR221
• Backbone size w/o insert (bp): 4762
• Total vector size (bp): 6556
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ACE2
• Species: H. sapiens (human)
• Insert Size (bp): 1794
• Mutation: Amino acids 19-615, No stop codon
• GenBank ID:
• Entrez Gene: ACE2 (a.k.a. ACEH)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned from pENTR223 ACE2, open (Addgene #149719) received from Addgene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA