pDONR221-ACE2 receptor 19-615
(Plasmid
#167012)
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PurposeGateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 167012 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepDONR221
- Backbone size w/o insert (bp) 4762
- Total vector size (bp) 6556
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameACE2
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SpeciesH. sapiens (human)
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Insert Size (bp)1794
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MutationAmino acids 19-615, No stop codon
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GenBank ID
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Entrez GeneACE2 (a.k.a. ACEH)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned from pENTR223 ACE2, open (Addgene #149719) received from Addgene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-ACE2 receptor 19-615 was a gift from Spyros Petrakis (Addgene plasmid # 167012 ; http://n2t.net/addgene:167012 ; RRID:Addgene_167012) -
For your References section:
AI Promoted Virtual Screening, Structure-Based Hit Optimization, and Synthesis of Novel COVID-19 S-RBD Domain Inhibitors. Gkekas I, Katsamakas S, Mylonas S, Fotopoulou T, Magoulas GEpsilon, Tenchiu AC, Dimitriou M, Axenopoulos A, Rossopoulou N, Kostova S, Wanker EE, Katsila T, Papahatjis D, Gorgoulis VG, Koufaki M, Karakasiliotis I, Calogeropoulou T, Daras P, Petrakis S. J Chem Inf Model. 2024 Nov 13. doi: 10.1021/acs.jcim.4c01110. 10.1021/acs.jcim.4c01110 PubMed 39535926