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pRc/RSV-m CBP-HA
(Plasmid #16701)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16701 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRc/RSV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CBP
  • Alt name
    CREB binding protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    8000
  • GenBank ID
    NP_001020603.1 NP_001020603.1
  • Entrez Gene
    Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer RSV promoter
  • 3′ sequencing primer BGH rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HA-tagged mouse CREB-binding-protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRc/RSV-m CBP-HA was a gift from Richard Goodman (Addgene plasmid # 16701 ; http://n2t.net/addgene:16701 ; RRID:Addgene_16701)

  • For your References section:

    Phosphorylated CREB binds specifically to the nuclear protein CBP. Chrivia JC, Kwok RP, Lamb N, Hagiwara M, Montminy MR, Goodman RH. Nature. 1993 Oct 28. 365(6449):855-9. 10.1038/365855a0 PubMed 8413673
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