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Addgene

pcDNA3. Flag-CSL V237A
(Plasmid #167007)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 167007 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.Flag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CSL
  • Species
    H. sapiens (human)
  • Mutation
    V237A
  • Entrez Gene
    RBPJ (a.k.a. AOS3, CBF-1, CBF1, IGKJRB, IGKJRB1, KBF2, RBP-J, RBP-J kappa, RBP-JK, RBPJK, RBPSUH, SUH, csl)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The WT CSL cDNA was kindly provided by Dr. Franz Oswald

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3. Flag-CSL V237A was a gift from Gian-Paolo Dotto (Addgene plasmid # 167007 ; http://n2t.net/addgene:167007 ; RRID:Addgene_167007)

  • For your References section:

    CSL controls telomere maintenance and genome stability in human dermal fibroblasts. Bottoni G, Katarkar A, Tassone B, Ghosh S, Clocchiatti A, Goruppi S, Bordignon P, Jafari P, Tordini F, Lunardi T, Hoetzenecker W, Neel V, Lingner J, Paolo Dotto G. Nat Commun. 2019 Aug 29;10(1):3884. doi: 10.1038/s41467-019-11785-7. 10.1038/s41467-019-11785-7 PubMed 31467287
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