pTJV1Sc3-rpoB
(Plasmid #166983)

• Purpose: Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
• Depositing Lab: Christopher Reisch
• Publication: Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac007. doi: 10.1093/synbio/ysac007. eCollection 2022.

Backbone

• Vector backbone: pTJV1Sc-rpoB
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6047
• Modifications to backbone: RiboJ and ribosome binding site added upstream of insert
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: RecT
• Alt name: EcRecT
• Species: E. coli
• Insert Size (bp): 807
• GenBank ID: NP_415865.1
• Entrez Gene: recT (a.k.a. b1349, ECK1346)
• Promoter: pVvanCC

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATTGGATCCAATTGACAGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry