pTJV1Sc2-tetA
(Plasmid
#166982)
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PurposeGenerates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166982 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTJV1Sc-rpoB
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCspRecT
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SpeciesCollinsella stercoris
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Insert Size (bp)810
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GenBank IDWP_006720782
- Promoter pVanCC
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer attggatccaattgacagc (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTJV1Sc2-tetA was a gift from Christopher Reisch (Addgene plasmid # 166982 ; http://n2t.net/addgene:166982 ; RRID:Addgene_166982) -
For your References section:
Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli. Ellington AJ, Reisch CR. Synth Biol (Oxf). 2022 May 3;7(1):ysac007. doi: 10.1093/synbio/ysac007. eCollection 2022. 10.1093/synbio/ysac007 PubMed 35673614
