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pTJV1ScKm-rpoB
(Plasmid #166980)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166980 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTJV1Sc-rpoB
  • Total vector size (bp) 6328
  • Vector type
    Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NeoR/KanR
  • Insert Size (bp)
    1429

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggcggatcttcacctagatc
  • 3′ sequencing primer gagacgttgatcggcacgt
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Same as pTJV1Sc-rpoB, but spectinomycin resistance marker replaced with kanamycin resistance

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTJV1ScKm-rpoB was a gift from Christopher Reisch (Addgene plasmid # 166980 ; http://n2t.net/addgene:166980 ; RRID:Addgene_166980)

  • For your References section:

    Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli. Ellington AJ, Reisch CR. Synth Biol (Oxf). 2022 May 3;7(1):ysac007. doi: 10.1093/synbio/ysac007. eCollection 2022. 10.1093/synbio/ysac007 PubMed 35673614
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