Skip to main content
Addgene

pSMT3-FBF2 AS/Y
(Plasmid #166974)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSMT3
  • Backbone manufacturer
    Chris Lima
  • Backbone size w/o insert (bp) 5633
  • Total vector size (bp) 6869
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FBF-2
  • Alt name
    FBF
  • Species
    C. elegans (nematode)
  • Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575; changed cysteine 363 to alanine, changed arginine 364 to tyrosine, changed glutamine 367 to serine
  • Entrez Gene
    fbf-2 (a.k.a. CELE_F21H12.5)
  • Promoter T7
  • Tag / Fusion Protein
    • His-SUMO (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSMT3-FBF2 AS/Y was a gift from Traci Hall (Addgene plasmid # 166974 ; http://n2t.net/addgene:166974 ; RRID:Addgene_166974)

  • For your References section:

    Engineering a conserved RNA regulatory protein repurposes its biological function in vivo. Bhat VD, McCann KL, Wang Y, Fonseca DR, Shukla T, Alexander JC, Qiu C, Wickens M, Lo TW, Tanaka Hall TM, Campbell ZT. Elife. 2019 Jan 17;8. pii: 43788. doi: 10.7554/eLife.43788. 10.7554/eLife.43788 PubMed 30652968
Related items: