XE115 pCS2+-E1A
(Plasmid
#16696)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16696 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepCS2+-EIA
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Description: An EcoR1 fragment of wild-type E1A (Bannister and Kouzarides,
1995, EMBO J. 14:4758-4762) was subcloned into pCS2+.
Notes for Use:
Prepared by: Ken-ichi Takemaru and Randall Moon, 2000, JCB 149:249-254.
human Ad5 E1A12S with M133L and A164V mutations
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE115 pCS2+-E1A was a gift from Randall Moon (Addgene plasmid # 16696 ; http://n2t.net/addgene:16696 ; RRID:Addgene_16696) -
For your References section:
The transcriptional coactivator CBP interacts with beta-catenin to activate gene expression. Takemaru KI, Moon RT. J Cell Biol. 2000 Apr 17. 149(2):249-54. 10.1083/jcb.149.2.249 PubMed 10769018