XE116 pCS2+-E1A mut CBP
(Plasmid
#16695)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16695 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeMammalian Expression ; Xenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepCS2+-EIA mut CBP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Description: An EcoR1 fragment of E1A, mutated (deletion of amino acids.
64-68) so it will not bind CBP, was subcloned into CS2+. The EcoR1 fragment.
was from E1AmutCBP described by Bannister and Kouzardies, 1995 (EMBO.
J. 14:4758-4762).
Notes for Use:
Prepared by: Ken-ichi Takemaru and Randall Moon, 2000, JCB 149:249-254.
human Ad5 E1A12S with L81F, M133L, and A164V mutations
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE116 pCS2+-E1A mut CBP was a gift from Randall Moon (Addgene plasmid # 16695 ; http://n2t.net/addgene:16695 ; RRID:Addgene_16695) -
For your References section:
The transcriptional coactivator CBP interacts with beta-catenin to activate gene expression. Takemaru KI, Moon RT. J Cell Biol. 2000 Apr 17. 149(2):249-54. 10.1083/jcb.149.2.249 PubMed 10769018