SiT-EGFP
(Plasmid
#166943)
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Purposemammalian expression of N' Sialyl Transferase tagged with EGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneEGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSialyl Transferase N-terminus
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SpeciesH. sapiens (human)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SiT-EGFP was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 166943 ; http://n2t.net/addgene:166943 ; RRID:Addgene_166943) -
For your References section:
Transport through the Golgi apparatus by rapid partitioning within a two-phase membrane system. Patterson GH, Hirschberg K, Polishchuk RS, Gerlich D, Phair RD, Lippincott-Schwartz J. Cell. 2008 Jun 13;133(6):1055-67. doi: 10.1016/j.cell.2008.04.044. 10.1016/j.cell.2008.04.044 PubMed 18555781
