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Addgene

GFP-p58
(Plasmid #166942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166942 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    GFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p58
  • Alt name
    ERGIC-53
  • Alt name
    LMAN1
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Lman1 (a.k.a. p58)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GFP (N terminal on insert)
    • chicken lysozyme signal peptide (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note there are two mutations in p58-L405Q, R419S Depositor confirms these do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-p58 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 166942 ; http://n2t.net/addgene:166942 ; RRID:Addgene_166942)

  • For your References section:

    Maintenance of Golgi structure and function depends on the integrity of ER export. Ward TH, Polishchuk RS, Caplan S, Hirschberg K, Lippincott-Schwartz J. J Cell Biol. 2001 Nov 12;155(4):557-70. doi: 10.1083/jcb.200107045. Epub 2001 Nov 12. 10.1083/jcb.200107045 PubMed 11706049
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