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Addgene

pB-01
(Plasmid #166924)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166924 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMA
  • Backbone manufacturer
    GeneArt - Thermo Fisher
  • Backbone size w/o insert (bp) 2456
  • Total vector size (bp) 2606
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTAC promoter - Block B- Flanked by BsaI restriction sites.
  • Alt name
    PTAC
  • Species
    Synthetic
  • Insert Size (bp)
    78

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TTGTAAAACGACGGCCAG
  • 3′ sequencing primer CATGGTCATAGCTGTTTCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-01 was a gift from Thomas Gorochowski (Addgene plasmid # 166924 ; http://n2t.net/addgene:166924 ; RRID:Addgene_166924)

  • For your References section:

    Harnessing the central dogma for stringent multi-level control of gene expression. Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE. Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7. 10.1038/s41467-021-21995-7 PubMed 33741937
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