pTYF-sGFAP-LOx-IRES-tdTomato
(Plasmid #166919)

• Purpose: Express the bacterial lactate oxidase (LOx) enzyme in astrocytes
• Depositing Lab: Sergey Kasparov
• Publication: Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.23960.

Backbone

• Vector backbone: pTYF
• Backbone size w/o insert (bp): 12266
• Total vector size (bp): 13392
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lactate oxidase
• Alt name: LOx
• Species: Synthetic
• Insert Size (bp): 1125
• GenBank ID: D50611.1
• Promoter: sGFAP
• Tag / Fusion Protein:
  • tdTomato (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (destroyed during cloning)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: GFAP middle-end (ATGGGTGAGGGGAGAGCT)
• 3′ sequencing primer: IRES reverse (CCAAGTCAGTGGCTGCAC)

Resource Information

• Supplemental Documents:
  • pTYF-sGFAP-Loxav-I-tdTom.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry