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pCDNA3_ReBphP-PCM_IRES_sfGFP
(Plasmid #166917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166917 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.0
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 8340
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ReBphP-PCM
  • Species
    Rhizobium etli
  • Insert Size (bp)
    1575
  • GenBank ID
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer gcgtctaagttacgggaag
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    sfGFP
  • Alt name
    superfolder GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    720
  • GenBank ID
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Notl (unknown if destroyed)
  • 5′ sequencing primer gactactttgggtgtccg
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ReBphP-PCM is codon-optimized for mammalian expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3_ReBphP-PCM_IRES_sfGFP was a gift from Andre Stiel (Addgene plasmid # 166917 ; http://n2t.net/addgene:166917 ; RRID:Addgene_166917)

  • For your References section:

    Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins. Mishra K, Stankevych M, Fuenzalida-Werner JP, Grassmann S, Gujrati V, Huang Y, Klemm U, Buchholz VR, Ntziachristos V, Stiel AC. Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi: 10.1126/sciadv.aaz6293. eCollection 2020 Jun. 10.1126/sciadv.aaz6293 PubMed 32582850
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