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Addgene

Halo-p58
(Plasmid #166896)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166896 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Halo
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p58
  • Alt name
    ERGIC-53
  • Alt name
    LMAN1
  • Species
    R. norvegicus (rat)
  • Mutation
    L405Q, R419S- please see depositor comments
  • Entrez Gene
    Lman1 (a.k.a. p58)
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms mutations in p58 (L405Q, R419S) do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Halo-p58 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 166896 ; http://n2t.net/addgene:166896 ; RRID:Addgene_166896)

  • For your References section:

    ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER. Weigel AV, Chang CL, Shtengel G, Xu CS, Hoffman DP, Freeman M, Iyer N, Aaron J, Khuon S, Bogovic J, Qiu W, Hess HF, Lippincott-Schwartz J. Cell. 2021 Apr 29;184(9):2412-2429.e16. doi: 10.1016/j.cell.2021.03.035. Epub 2021 Apr 13. 10.1016/j.cell.2021.03.035 PubMed 33852913
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