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Addgene

Halo-Sec31
(Plasmid #166895)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166895 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Halo-C
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec31
  • Species
    H. sapiens (human)
  • GenBank ID
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Halo-Sec31 was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 166895 ; http://n2t.net/addgene:166895 ; RRID:Addgene_166895)

  • For your References section:

    ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER. Weigel AV, Chang CL, Shtengel G, Xu CS, Hoffman DP, Freeman M, Iyer N, Aaron J, Khuon S, Bogovic J, Qiu W, Hess HF, Lippincott-Schwartz J. Cell. 2021 Apr 29;184(9):2412-2429.e16. doi: 10.1016/j.cell.2021.03.035. Epub 2021 Apr 13. 10.1016/j.cell.2021.03.035 PubMed 33852913
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