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Addgene

VAMP2-pHmScarlet
(Plasmid #166890)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166890 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    EGFP-N1
  • Modifications to backbone
    EGFP was replaced with pHmScarlet
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VAMP2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Vamp2 (a.k.a. Syb-2, Syb2, Vamp-2, sybII)
  • Promoter CMV
  • Tag / Fusion Protein
    • pHmScarlet (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VAMP2-pHmScarlet was a gift from Pingyong Xu (Addgene plasmid # 166890 ; http://n2t.net/addgene:166890 ; RRID:Addgene_166890)

  • For your References section:

    pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis docking and fusion steps. Liu A, Huang X, He W, Xue F, Yang Y, Liu J, Chen L, Yuan L, Xu P. Nat Commun. 2021 Mar 3;12(1):1413. doi: 10.1038/s41467-021-21666-7. 10.1038/s41467-021-21666-7 PubMed 33658493
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