pRK5 mFz8CRD-IgG
(Plasmid #16689)

• Depositing Lab: Xi He
• Publication: Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Frizzled-8
• Alt name: mfz8
• Alt name: Fz8
• Species: M. musculus (mouse)
• Mutation: CRD domain of mFz8
• Entrez Gene: Fzd8 (a.k.a. Fz8)
• Tag / Fusion Protein:
  • IgG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mFz8-CRD-IgG was originally constructed by Jeremy Nathans at Johns Hopkins/HHMI.
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the CRD domain of mouse Frizzled-8 as an IgG fusion.