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Addgene

XE68 APC-GFP in CS2+
(Plasmid #16687)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16687 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APC-GFP
  • Alt name
    apc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3100
  • Entrez Gene
    APC (a.k.a. BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Cla1 (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fragment of human APC (aa 1014-1058) that contains the three 15 aa Beta-catenin binding sites and the seven 20 aa Beta-catenin binding sites. Addgene QC found 3 point mutations that do not affect function: S1205N, T1292S, and V1822D.

Linearize with Not 1 and transcribe with SP6.

Plasmid History:
Constructed by Jeffery Miller.
Species of Sequence Origin: human.
Reference: Miller and Moon, 1997, J. Cell Biology 139:229-244.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE68 APC-GFP in CS2+ was a gift from Randall Moon (Addgene plasmid # 16687 ; http://n2t.net/addgene:16687 ; RRID:Addgene_16687)

  • For your References section:

    Analysis of the signaling activities of localization mutants of beta-catenin during axis specification in Xenopus. Miller JR, Moon RT. J Cell Biol. 1997 Oct 6. 139(1):229-43. 10.1083/jcb.139.1.229 PubMed 9314542
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