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Addgene

pGS-21a his6-GST-WTPhi29DNAP
(Plasmid #166863)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 166863 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGS-21a
  • Backbone manufacturer
    GenScript
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 DE3 for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Phi29 Polymerase codon-optimized for overexpression in E. coli
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • his6 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGS-21a his6-GST-WTPhi29DNAP was a gift from Paul Blainey & Stephen Quake (Addgene plasmid # 166863 ; http://n2t.net/addgene:166863 ; RRID:Addgene_166863)

  • For your References section:

    Digital MDA for enumeration of total nucleic acid contamination. Blainey PC, Quake SR. Nucleic Acids Res. 2011 Mar;39(4):e19. doi: 10.1093/nar/gkq1074. Epub 2010 Nov 11. 10.1093/nar/gkq1074 PubMed 21071419

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