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Addgene

Xenopus APC-myc
(Plasmid #16686)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16686 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone size w/o insert (bp) 4350
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APC
  • Alt name
    XAPC
  • Alt name
    adenomatous polyposis coli
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    8500
  • Entrez Gene
    apc.L (a.k.a. XELAEV_18008006mg, apc, xapc)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: Full length Xenopus APC with an amino terminal c-myc epitope in the vector pCS2+MT.

Insert size: ~8.5kb. NcoI site introduced at 5' end. Linearize with Not1 and transcribe with SP6.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Xenopus APC-myc was a gift from Barry Gumbiner (Addgene plasmid # 16686 ; http://n2t.net/addgene:16686 ; RRID:Addgene_16686)

  • For your References section:

    Adenomatous polyposis coli tumor suppressor protein has signaling activity in Xenopus laevis embryos resulting in the induction of an ectopic dorsoanterior axis. Vleminckx K, Wong E, Guger K, Rubinfeld B, Polakis P, Gumbiner BM. J Cell Biol. 1997 Jan 27. 136(2):411-20. 10.1083/jcb.136.2.411 PubMed 9015311
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