pBbB8k-csg-NbGFP
(Plasmid
#166858)
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PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 166858 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBb
- Backbone size w/o insert (bp) 3763
- Total vector size (bp) 7273
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namecsgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
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Insert Size (bp)3519
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.11.23.394965v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBbB8k-csg-NbGFP was a gift from Neel Joshi (Addgene plasmid # 166858 ; http://n2t.net/addgene:166858 ; RRID:Addgene_166858) -
For your References section:
Hybrid Living Capsules Autonomously Produced by Engineered Bacteria. Birnbaum DP, Manjula-Basavanna A, Kan A, Tardy BL, Joshi NS. Adv Sci (Weinh). 2021 May 3;8(11):2004699. doi: 10.1002/advs.202004699. eCollection 2021 Jun. 10.1002/advs.202004699 PubMed 34141524
Map uploaded by the depositor.
