SARS CoV-2 RBD (bio-His)
(Plasmid #166857)

• Purpose: Human expression vector encoding SARS CoV-2 receptor binding domain (RBD) (amino acids 319-541), containing biotinylation site and 6His tag.
• Depositing Lab: Evelien Bunnik
• Publication: Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. doi: 10.1371/journal.pone.0261656. eCollection 2021.

Backbone

• Vector backbone: pTT3
• Total vector size (bp): 6720
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS CoV-2 receptor binding domain (RBD)
• Species: Severe acute respiratory syndrome-related coronavirus
• Entrez Gene: S (a.k.a. GU280_gp02, spike glycoprotein)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHis (C terminal on insert)
  • Biotinylation site

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: .

Resource Information

• A portion of this plasmid was derived from a plasmid made by: BEI NR-52309
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid modified and submitted by Sebastiaan Bol, Raphael Reyes, Katie Clarke and Evelien Bunnik. Biotin ligase BirA and free biotin are required for biotinylation of the biotinylation site. Expression confirmed by SDS-PAGE and Western blot (anti-biotin and anti-His).